Insights Into Monitoring Changes in the Viable Cell Density and Cell Physiology using Scanning, Multi-Frequency Dielectric Spectroscopy

December 4, 2013

 

Carvell, J., L.J. Graham, and B.J. Downey, “Insights Into Monitoring Changes in the Viable Cell Density and Cell Physiology using Scanning, Multi-Frequency Dielectric Spectroscopy,” BMC Proceedings 2013, 7:Suppl. 6(2013)4.

 

  • Dielectric spectroscopy (DS) is now the most common method for estimating the in situ live cell concentration in animal cell culture.
  • DS and traditional offline methods for cell counting based on Trypan Blue correlate well during the growth phases but with some cell lines, deviations are observed during the late growth phase.
  • Scanning multi-frequency DS can detect the physiological changes of the cells during the death phase of the culture including changes in cell size, membrane capacitance and internal conductivity.
  • The concept of using the Area Ratio Algorithm (ARA) looks to be a relatively simple and promising method for providing on-line cell counts that correlate well with traditional methods for the complete cell growth cycle.

 

The work presented here shows the utility of frequency scanning data to obtain enhanced measurement of VCV using non-invasive capacitance sensors in reusable and single use bioreactors. The information-rich nature of dielectric frequency scanning allows interrogation of biophysical properties of cells. The concept can be extended to samples off-line.

 

 

 

Topics: 
Biotherapeutics / Bioprocessing